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Research Experience

April 17, 2017 - April 23, 2018: Visiting Scientist under CAS-PIFI Fellowship on the effect of biogenic silver nanoparticles on the glioblastoma stem cells in vitro and in vivo.

Jun 2005 - Feb 2007: JSPS Postdoctoral Fellowship on Refinement of protein-carbohydrate interaction and its application to protein remodeling at the Laboratory of Functional Biomolecules, Graduate School of Life Science, Tohoku University, Sendai, Japan.

Oct 2000 - Mar 2004: Research and Doctoral Student under Monbusho Scholarship on the Structural studies of actin and acto-S1 in different stages (Ph. D dissertation: Hydration study on Actin and Acto-S1 (Myosin subfragment-1) in solution by microwave dielectric spectroscopy) at the laboratory of physical chemistry of Biomolecules, Dept. of Material Sciences, Graduate School of Engineering, Tohoku University, Sendai, Japan.

M. Sc. dissertation: Purification, Characterization and Structure-function relationship of Rice Bran Lectins.

The key strengths 
• PhD in protein science with 20+ years experience in animal, plant & recombinant protein biochemistry, 14+ years in molecular biology techniques, 14+ years in cell biology and oncology (cell culture, apoptosis mechanism study & mice handling).
• Design, develop and execute multi-disciplinary research strategies.
• Data analysis, manuscript preparation & submission to leading journals.
• Manage scientific and professional relationships and collaboration.
• Prepare slides and fluently deliver lectures in classes and scientific conferences.
• Working experience in the multidiscipline field and multi-culture environment.
• Supervise Ph.D. & M.Sc. level students and guide research team.

Expertise
Cell & Molecular Biological Techniques: Human glioblastoma stem cell and other cancer cells (MCF-7, HCT-116, HeLa, A549, SU87, BXPC-3, T98G, U937 & Skov-3) culture, toxicity assay, cell staining (with Hoechst 33342, Dapi, propidium iodide, trypan blue, annexin V etc.), detection of proteins by immunofluorescence assay, cell cycle analysis by Flow cytometry.
Isolation of DNA & RNA, Expression of genes by RT PCR and Real-time PCR, agarose gel electrophoresis, and Knowledge on CRISPR-Cas9.
Ehrlich ascites carcinoma cells bearing Swiss mice, WBC and RBC count, Hemoglobin
measurement, detection of urea, SGPT, SGOT, total protein, alkaline phosphatase, glucose, and
triglyceride detection.
Protein Purification & related techniques: Gel filtration, Ion exchange, hydrophobic and affinity
chromatography, SDS-PAGE, western blotting. sequence determination, amino acid analysis, glycoprotein
detection, sugar separation and identification, and crystal preparation. Expression of recombinant actin in Dictyostelium discoideum cells, analysis of biomolecules and enzymes.
Characterization of nanoparticles: UV-visible spectroscopy, AFM, TEM, TGA, FTIR, SEM, XRD.
Computer & Bioinformatics: Molecular docking, MS-Office, Protein Blast, XEI for AFM, Adobe Photoshop CS, RasMol, SPSS, GraphPad prism, FloMax, FlowJo, CytExpert 2.3. and QualX2.0 software.

Research Fields

Protein Biochemistry, Cancer Biology, Anticancer drug discovery, Nanomedicine, Molecular Biology.     

Research interests

Cancer is one of the leading causes of death worldwide. For the treatment of cancer besides surgery, radiation therapy, hormone therapy, immunotherapy, chemotherapy etc. are used. Chemotherapy and some other techniques have severe side effects and to solve the problems, scientists are trying to find out alternative ways and less toxic and more effective drugs. For those purposes, several therapeutic proteins, nanoparticles, and small molecules are now using in this laboratory to check anticancer activity against breast, colorectal, lung and hepatocellular carcinoma, cervical cancer cell lines The potent anticancer agents are subjected for the elucidation of molecular mechanism against the cancer cell lines. Finally, the effective samples are used against the very rapidly growing Ehrlich ascites carcinoma cells in vivo in Swiss albino mice.   

Scientific contributions 

(1) During Ph.D studentship in Japan, hypermobile water around the actin and acto-myosin subunit-1 was discovered for the first time that plays a great role in the chemo-mechanical energy transduction during muscle contraction (Biophysical Journal, 2003, 85: 3154–3161).  

(2) Several novel lectins were purified from plant sources at our laboratory at the University of Rajshahi. Some of them showed potent anticancer effects against Ehrlich ascites carcinoma cell and human cancer cell lines. Anticancer mechanism-related research at the molecular level (cell cycle analysis, study of apoptosis by fluorescence microscope and expression of apoptosis-related genes) was started first time in Bangladesh by our research group (led by me) (Ref. Phytomedicine 2013, 15;20(14):1288-96, on the basis of PubMed). 

(3) In the council meeting of the 5^th Asia Pacific Protein Association (APPA) conference in Thailand, it became possible for me to convince the councilors of APPA due to the great effort of our research group (led by me). As a result, Bangladesh got the full membership status of APPA.

(4). Biogenic silver nanoparticles-related research work has also been started for the first time in Bangladesh (Journal of Nanoparticles. Volume 2016, Article ID 6898926) by our research group (led by me)  and the article (Kabir et al., Journal of Molecular and Cellular Medicine, 2020, 24(22):13223-13234) representing the first paper in the world on the effect of biogenic silver nanoparticles against any stem cancer cells (On the basis of PubMed). 

Academic Awards

1. Deans Award-2022 in the “Biochemical and Chemical” section from the Faculty of Science, University of Rajshahi..

2. UGC (University Grants Commission, Bangladesh) Gold Medal Award-2016 (Previously UGC Award) in the fields of Life Sciences.

3. CAS-PIFI Fellowship (Chinese Academy of Science) as Visiting Scientist.

4. Deans Award-2016 in the “Biochemical and Chemical” section from the Faculty of Science, University of Rajshahi.

5. UGC (University Grants Commission, Bangladesh) Award-2011 in the fields of Clinical,      Pharmaceutical and Medical Sciences.

6. JSPS Postdoctoral Fellowship (Government of Japan)

7. MONBUSHO Scholarship (Government of Japan).

8. University Award for the best result in M.Sc Examination (University of Rajshahi).

9. University Award for the best result in B.Sc (Honors) Examination (University of Rajshahi).

10. Shaheed Suhrawardy Hall Gold Medal for the best result in B.Sc (Honors) Examination (University of Rajshahi).

 

Supervision of Ph.D degree

1. Purification, Characterization and studies of biological activities of a novel lectin from Nymphaea nouchali tuber. Awardees Name: Md. Abu Zubair, Ph.D awarded 2014. (Supervisor)

2. Isolation, characterization and antiproliferative activities of a lectin from Kaempferia rotunda rhizome.Awardees Name: Fazle Rabbi Shakil Ahmed, Ph. D awarded 2017. (Supervisor)

3. Antiproliferative activity of Sword bean lectin and carbonate apatite against Ehrlich ascites carcinoma cell. Awardees Name: Momtaz Fatima Waheed, Ph. D awarded 2017. (Co-supervisor)

4. Purification and characterizations of a new mannose-specific lectin from Bangladeshi cultivated sword bean with antiproliferative activities against Ehrlich ascites carcinoma cells in vivo in mice. Awardees Name: Mst. Jesmin Sultana, Ph. D awarded 2017. (Co-supervisor)

5. Synthesis of biogenic silver/silver chloride nanoparticles from seaweed (Hypnea musciformis) and its effects on pathogenic bacteria and human cancer cell lines. Dissertation submitted by Rita Ghose. (Supervisor)

Supervision of M.Phil degree

1. Studies on biological properties of chitin-binding lectins from potato (Solanum tuberosum) species cultivated in Bangladesh. Awardees Name: Imtiaj Hasan, Awarded 2013. (Supervisor)

2. A new lectin from the Pulp of Punica granatum: Isolation, characterization and antiproliferative activities. Awardees Name: Md. Nurujjaman, Awarded 2017. (Co-supervisor)

 

Supervision of M.Sc Thesis (with the date of submission)

1. Purification, Characterization of a Lactin from the white water lily. (Nymphaea nouchali) Tuber. Submitted by: Md. Nurujjaman in June, 2009.

2. Study on Biological and Physico-Chemical Parquetries of Lectins from water chestnut (Trapabispinosa). Submitted by: Md. Azizul Haque (Helal) in January, 2010

3. Physico-Chemical, Antimicrobial Cytotoxie study of a lectin from Snake Gourd (Trichosasthes Cucumerina) Seeds.  Md. Jahangir Alam in October, 2010.

4. Antitumor and Antimicrobial study with physico-Chemical Characterization and structural analysis of a novel lectin from Tuberous Rhizome of Kaempferia rotunda.

Submitted by: Md. Amir Hossain in August, 2011.

5. Purification and Characterization of a Chitinbinding Protein from Pointed gound seeds (Trichosanthes dioica). Submitted by: Most. Shahnima Tasnim in April 2014.

6. Purification, Characterization, Antimicrobial, Cytotoxic and Structural stability study of a lectin from pointed gound (Trichosanthes dioica)  seeds cultivated in Bangladesh. Submitted by: Md. Rezaul Karim in April 2014 (Joint Supervision).

7. Purification, Characterization of a lectin from the Pulp of Pomegranate (Punica granatum). Submitted by: Tanjila Mashhoor in April 2015.

8. Trichosanthes dioica Seed Lectin (TDS)  Inhibits Ehrlich Ascites Carcinoma (FAC) Cell Growth by Inducing Go/Gi Cell Cycle Arrest.

Submitted by: Shaikh Shohidul Islam in April 2015

9. Purification, Characterization of a Chitinase from Pointed gourd (Trichosanthes dicica) seeds cultivated in Bangladesh. Submitted by: Nazma Khatun in April 2015.

(Joint Supervision)

10. Investigation of biological properties and structural stability of purified Lectin from moringa oleifera seeds.

Submitted by: Aninda Chakraborty in September 2015.

11. Antibacterial and antiproliferative activities of silver nanoparticles from Fragaria ananassa (Strawberry) Fruit juice.  Submitted by: Md. Musfiqur Rahman in September 2015.

12. Purification, Characterization of a novel Lectin from moringa oleifera seeds cultivated in Bangladesh. Supervisor: Submitted by: Mst. Sadnima Zaman in September 2015. (Joint Supervision)

13. Green synthesis of silver nanoparticles from Averrhoa carambola (Star fruit) Extract and its Antibacterial and antiproliferative activities.  M. S. Haque in September 2015. (Joint Supervision)

14. Purification of Characterization of a novel Lectin from Carissa carandas seeds. Submitted by: Md. Golam Kibria in August 2016. (Joint Supervision).

15. Antibacterial and antiproliferative activities of silver nanoparticles from Citrus sinensis (Orange) fruit juice.

Submitted by: Roksana Ferdosh in August 2016. (Joint Supervision).

16. Study of actinobacterial activity of Biogenic silver nanoparticles from citrus lemon extract and its anticancer activity against Ehrlich ascites carcinoma cells in August 2017. (Joint Supervision).

17. Isolation of a lectin with antibacterial activity from Geodorum densiflorum rhizome. Submitted by: K.M. Ahshanul Kabir in August 2017. (Joint Supervision).

18. Purification and Characterization of a Novel Lectin from Asparagus racemosus Rhizome with Antiproliferative Activities against Ehrlich Ascites Carcinoma Cells. Submitted by: Jahanur Islam in June 2019.

19. Partial purification and characterization of a lectin from Lens culinaris seeds and study of it’s antibacterial properties. Submitted by Md. Mahenur Islamin October 2020.

20. Purification, characterization and study of antibacterial activity of a novel lectin from Amaranthus gangeticus seeds. Submitted by Sharif Nasibur Rahman in October 2020. (Joint Supervision). 
21. Partial purification, characterization and antimicrobial activity of a lectin from garlic (Allium sativum) bulb cultivated in Bangladesh. Submitted by Sourov Kumar Ghosh in October 2020.

22. An Adjuvant Comparative Antimicrobial and Antiproliferative Study of Kaempferia rotunda and Zizyphus mauritiana-mediated Silver/Silver Chloride Nanoparticles Focusing on EAC, HCT-116, A-549, MCF-7 Cell Lines, and Toxicity Against Brine Shrimp Nauplii. Submitted by Md Mahmudul Hasan in February 2022.

23. An Adjuvant Comparative Antimicrobial , Antioxidant and Toxicity Against Brine Shrimp Nauplii Study of Geodorum densiflorum and Asparagus racemosus -mediated Silver/Silver Chloride Nanoparticles. Submitted by Sharmin Sultana in September 2022.

Membership of professional organizations

• 1.	Council member of the Asia Pacific Protein Association (APPA)
  2.	American Chemical Society.
  3.	Protein Society of Thailand.
  4.	Bangladesh Crystallographic Association (Life and former executive committee member).
  5.	The Bangladesh Society for Biochemistry and Molecular Biology (Life Member).
  6.	The Bangladesh Society of Microbiologists (Life Member).
  7.	Bangladesh Academy of Advance Sciences (Life Member).
  8.	The Bangladesh JSPS Alumni Association (Life Member).
  9.	Bioinformatics Research Group of the University of Rajshahi (General Member).
  10.	Japanese Biophysical Society (Former Member).

   

  List of the funded research projects (Completed)

  1. Syed Rashel Kabir. Synthesis of protein-coated silver nanoparticles and evaluation of its anticancer mechanism against human lung, breast, and colorectal cancer cell lines. No: 303/5/52/RU/Science-30/2021-2022. 

  2. Syed Rashel Kabir and Farhadul Islam. Elucidation of the anticancer mechanism of lectins against human breast lung and hepatocellular carcinoma cells. Ministry of Science and Technology, Bangladesh. Number of sanction order: 09.00.0000.009.14.019.21-745/560-MS. 2021-2022.

  3. Syed Rashel Kabir. “Elucidation of the mechanism of antiproliferative activities of proteins against human breast, colorectal and mice cancer cell lines.” Grant ID No. 1225/5/52/RU/Science-25/ 2019-2020.

  4. Syed Rashel Kabir. “Therapeutic evaluation of plant lectins as anticancer agents against different cancer cell lines. Number of sanction order:” 39.00.0000.009.06.024.19/MEDI’S-505. 2019-2020.

  5. Syed Rashel Kabir and Asaduzzaman A.K.M. “Green synthesis of silver 4.. nanoparticles and its therapeutic evaluation as anticancer and antimicrobial agent”, Ministry of Science and Technology, Bangladesh. Number of sanction order: 39.009.002.01.00.057.2015- 2016/922/Phys-362 2015-2016.

  6.  Syed Rashel Kabir and  Hossain M T ”Investigation of lectins as potent inducers of apoptosis in cancer cells”, Ministry of Science and Technology, Bangladesh. Number and date of sanction order:39.009.002.01.00.053.2014-2015 16/MEDI;S-165. 2014-2015.

  7. Syed Rashel Kabir and Uddin M B ”Elucidation of anticancer properties of a plant lectin”, Faculty of Science, University of Rajshahi, Bangladesh. 2014-2015.

  8. Syed Rashel Kabir and Imtiaj Hasan “Antimicrobial and anticancer studies of lectins isolated from plant sources cultivated in Bangladesh”, Ministry of Science and Technology, Bangladesh. Number and date of sanction order: 39.009.006.01.00.042.2012-2013/BS-67. 2012-2013.

  9. Syed Rashel Kabir “Study on biological effects and pharmaceutical applications of lectins”, Rajshahi University, Bangladesh. 2011-2012.

  10. Syed Rashel Kabir, “Development of the rapid chromatographic method for a lectin purification and study on its characterization, toxicity and pharmacological usefulness”. University Grant Commission (UGC), Bangladesh Project No. Life/2007-8/8498. 2007-2008.

  11. Syed Rashel Kabir, “Establishment of the single-step purification technique for a lectin and study on the Physico-chemical, pharmacological and antimicrobial properties of the purified lectin.” Rajshahi University, Bangladesh. 2007-2008

  
  

  Conference abstract
  

  124 conference abstracts were published among them 5 as an invited speaker, 9 oral presentations in international conferences, 12 oral presentations in national conferences, 49 poster presentations in international conferences and 47 poster presentations in national conferences.

  Presentation as Invited speaker

  1. Plant lectins inhibit human colorectal cancer cell growth by inducing apoptosis in several pathways. Technical Session: Biochemistry-2, International Conference on Science and Technology for Celebrating the Birth Centenary of Bangladesh (ICSTB-2021). March 11-13, 2021, Venue: Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka. Bangladesh

  2. Therapeutic evaluation of lectins and green nanoparticles as promising anticancer agents. Bangladesh Society for Biochemistry and Molecular Biology conference, 27-28 April 2019, Venue: Dept. of Biochemistry and Molecular Biology, University of Chittagong, Bangladesh.

  3. Therapeutic evaluation of plant lectins as promising anticancer agents. The 5th Asia Pacific Protein Association Conference (APPA) and The 12th International Symposium of the Protein Society of Thailand, July 11-14, 2017, The Tide Resort, Bangsaen, Thailand.

  4. Therapeutic evaluation of lectins and green nanoparticles from plant sources as anticancer and antimicrobial agents. India Bangladesh Structural Conference, Sep 18-19, 2015 IISER, Kolkata, India.

  5. Momordica charantia seeds lectin showed antitumor activity through G₀/G₁ cell cycle arrest in vivo in mice. “Science, technology and Innovative technologies in the prosperous epoch of the powerful state”, June 12 -14, 2014 at the Academy of Sciences of Turkmenistan.

  
  

  • Associate Editor
  • Journal: Frontiers in Drug Discovery ( section "Anti-Cancer Drugs")

  
  

  Guest Editor 
  

  Journal: "Frontiers in Chemistry" (Impact Factor 5.221). 

Section: Medicinal and Pharmaceutical Chemistry section 

Editorial Board Member

Journal of Solid Tumors (Canada)

Electronic Journal of Biology (Germany)

Journal of Biochemistry and Molecular Biology Research (Hong Kong)

Manuscripts reviewed of the following 64 SCI category journals (Impact Factor, Publisher)

 

Cell Proliferation (8.75, Wiley), International Journal of Biological Macromolecules (8.02, Elsevier), Materials Science and Engineering: C (7.32, Elsevier), International Journal of Nanomedicine (7.03, Dove Medical Press), Bioengineered (6.8, Taylor & Francis), Life Sciences (6.78, Elsevier), Phytomedicine (6.65, Elsevier), International Journal of Pharmaceutics (6.51, Elsevier), Frontiers in Molecular Biosciences (6.113, Frontiers), Arabian Journal of Chemistry (6.0, Elsevier), International Immunopharmacology (5.71, Elsevier), Frontiers in Chemistry (5.545, Frontiers), Nanoscale Research Letters (5.41, Springer), Journal of Ethnopharmacology (5.19, Elsevier), Chemico-Biological Interactions (5.15, Elsevier), Journal of Drug Delivery Science and Technology (5.0, Elsevier), Applied Microbiology and Biotechnology (IF 5.0, Springer), Process Biochemistry (4.885, Elsevier), Cancer Medicine (4.71, Wiley), Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology (4.52, Elsevier), Journal of Cellular Biochemistry (4.48, Wiley), Drug Design, Development and Therapy (4.16, Dove Medical Press), AMB Express (4.126, Springer), Applied Organometallic Chemistry (4.07, Wiley), Toxicology Mechanisms and Methods (4.01, Taylor & Francis), Bioscience Reports (3.97, Springer), BMC Complementary Medicine and Therapies (3.9, Springer Nature), Microbial Pathogenesis (3.84, Elsevier), Drug Development Research (3.8,Wiley), Heliyon (3.77, Cell press), Medical Oncology (3.73, Springer), Journal of Food Biochemistry (3.65, Wiley), International Journal of Food Science and Technology (3.61, Wiley), Cell Biochemistry & Function (3.6, Wiley), BBRC (Elsevier, 3.57) Journal of Cluster Science (3.44, Springer), Microscope and Micro Analysis (3.41, Cam. Uni. Press), Biomed Research International (3.24, Hindawi),  Acta Tropica (3.22, Elsevier), South African Journal of Botany (3.11, Elsevier), Applied Biochemistry and Biotechnology (3.09, Springer), Integrative Cancer Therapies (3.07, SAGE), Toxicon (3.05, Elsevier),  BBA-General Subjects (3.0, Elsevier), Clinical and Experimental Pharmacology and Physiology (2.96, Wiley), Molecular Biology Reports (2.8 Springer Nature SNAPP), Journal of Stored Products Research (2.78, Elsevier), Chemical Physics Letters (2.719, Elsevier),  Archives of Oral Biology (2.64, Elsevier),  Journal of Medicinal Food (2.54, Mary Ann Liebert, Inc), Journal of Nanoparticle Research (2.53, Springer), Journal of Herbal Medicine (2.5, Elsevier), Molecular Biotechnology (2.5, Springer), Dose-Response (2.5, Sage Pub.) Natural Product Research (2.48 Taylor & Francis), Journal of Venomous Animals and Toxins including Tropical Diseases (2.4, Wiley), Journal of Chemotherapy (2.38, Taylor & Francis), Reproductive Biology (2.08, Elsevier), Flora (2.22, Elsevier), Protein & Peptide letters (1.92, Bentham Science), Natural Product Communications (1.8, Sage Pub), Saudi Medical Journal (1.6), Journal of Ayurveda and Integrative Medicine (1.2, Elsevier). 

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